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Research Tools

🧪 Peptide Reconstitution Calculator

Free laboratory calculator for research use. Results display instantly in-browser — no data is transmitted.

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About This Calculator

Reconstituting a lyophilised (freeze-dried) research peptide correctly is the first — and most critical — step in any laboratory protocol. Adding too little solvent produces a dangerously concentrated stock; too much dilutes it beyond the working range of most assays. This calculator removes the arithmetic entirely: enter the vial content and your target concentration and it returns the exact volume of bacteriostatic water (or sterile water) to add.

All OL Research peptides are supplied as lyophilised solids. Bacteriostatic water is the standard reconstitution solvent for most peptides — it contains 0.9% benzyl alcohol as a preservative, extending the usable life of the reconstituted solution to 28–30 days at 2–8°C. For short-term in-vitro use, sterile water for injection (WFI) is also appropriate.

Protocol note: introduce solvent slowly down the inner wall of the vial — never jet it directly onto the lyophilised cake. Swirl gently; do not vortex. Allow 2–3 minutes at room temperature for complete dissolution before use or aliquoting.

Calculator

Your required stock concentration
Result
—mL solvent to add
—Final concentration

Frequently Asked Questions

What solvent should I use to reconstitute research peptides?
Bacteriostatic water (0.9% benzyl alcohol) is the standard choice — it extends reconstituted peptide stability to 28–30 days at 2–8°C. Sterile water for injection is appropriate for immediate in-vitro use. Some hydrophobic peptides may require a small volume of glacial acetic acid or DMSO before dilution in aqueous buffer.
How long is a reconstituted peptide stable?
Reconstituted peptides in bacteriostatic water stored at 2–8°C are typically stable for 14–30 days. Frozen aliquots at −20°C are stable for 3–6 months; at −80°C, 12+ months. Avoid repeated freeze-thaw cycles — aliquot before freezing.
What concentration should I target?
1 mg/mL is the most common stock concentration for research peptides — it gives round-number volumes for most working concentrations and fits standard insulin syringes at useful dose ranges. Higher concentrations (e.g. 5 mg/mL) reduce solvent volume but can affect solubility for larger peptides.
Why should I not vortex the reconstituted peptide?
Vortexing creates air bubbles and mechanical shear that can denature peptide bonds and accelerate degradation. Gentle swirling is sufficient for dissolution.