🔬 Serial Dilution Calculator for Research Peptides

Serial dilutions are the backbone of dose-response experiments, IC50 determination, and concentration-response curves. A systematic dilution series generates a range of concentrations spanning several orders of magnitude from a single stock solution — typically 6–12 points across a 3–4 log range. Planning these manually introduces arithmetic errors; this calculator generates the complete series table including transfer volume and diluent volume per step.

The standard approach for a 1:10 dilution series: transfer 1 part stock into 9 parts diluent (e.g. 20 µL into 180 µL) to achieve a 10-fold reduction at each step. For a 1:3 series (half-log steps), transfer 1 part into 2 parts. The choice of dilution factor determines the resolution of your dose-response curve — 1:3 gives finer granularity; 1:10 covers a wider range per step.

For peptide dose-response experiments, start your highest concentration at 3–10× the expected IC50/EC50 and span at least 4 orders of magnitude. Use the molarity calculator to confirm your stock concentration before beginning the series.